Journal: Frontiers in Physiology
Article Title: Uncovering the mechanism of arecoline’s effect on oral submucous fibrosis: integrating transcriptomics and in vitro and in vivo experiments
doi: 10.3389/fphys.2026.1768602
Figure Lengend Snippet: Arecoline-induced fibrosis in hOMF cells. (A) Experimental design flowchart; (B) Effects of different doses of arecoline on hOMF cell viability at 24h ( x ¯ ±s, n = 3); (C) Effects of different doses of arecoline on hOMF cell viability at 48h ( x ¯ ±s, n = 3); (D) Effects of different doses of arecoline on hOMF cell viability at 72h ( x ¯ ±s, n = 3); (E) Immunofluorescence staining of α-SMA expression in hOMF cells treated with arecoline (n = 3); (200x); (F) Immunofluorescence staining of Col1a1 expression in hOMF cells treated with arecoline (n = 3); (200x); (G) Quantitative analysis of α-SMA fluorescence intensity ( x ¯ ± s , n = 3); (H) Quantitative analysis of Col1a1 fluorescence intensity ( x ¯ ±s, n = 3). (I) Protein expression levels of bFGF ( x ¯ ±s, n = 3); (J) Protein expression levels of TGF-β1 ( x ¯ ±s, n = 3); (K) Relative mRNA expression levels of YAP ( x ¯ ±s, n = 3); (L) Relative mRNA expression levels of TAZ ( x ¯ ±s, n = 3). Compared with the Control group, * P < 0.05, ** P < 0.01.
Article Snippet: Primers were designed by Wuhan Kingwell Biotechnology Co., Ltd. TGF-β (E-EL-0162) and bFGF (E-EL-H6042) ELISA kits, as well as the Enhanced Cell Counting Kit 8 (WST-8/CCK8) (E-CK-A362), were purchased from Elabscience Biotechnology (Wuhan, China).
Techniques: Immunofluorescence, Staining, Expressing, Fluorescence, Control